interleukin-1, tumor necrosis factor-alpha, and transforming growth factor-beta 1 and integrative meniscal repair influences on meniscal cell proliferation and migrationinterleukin-1、肿瘤坏死因子-α和转化生长因子1和综合半月板修复对半月板细胞增殖和迁移的影响.pdfVIP

Riera et al. Arthritis Research Therapy 2011, 13:R187 /content/13/6/R187 RESEARCH ARTICLE Open Access Interleukin-1, tumor necrosis factor-alpha, and transforming growth factor-beta 1 and integrative meniscal repair: influences on meniscal cell proliferation and migration 1 1,2 1,2 3,4 1,2* Katherine M Riera , Nicole E Rothfusz , Rebecca E Wilusz , JB Weinberg , Farshid Guilak and Amy L McNulty1 Abstract Introduction: Interleukin-1 (IL-1) and tumor necrosis factor-a (TNF-a) are up-regulated in injured and osteoarthritic knee joints. IL-1 and TNF-a inhibit integrative meniscal repair; however, the mechanisms by which this inhibition occurs are not fully understood. Transforming growth factor-b 1 (TGF-b 1) increases meniscal cell proliferation and accumulation, and enhances integrative meniscal repair. An improved understanding of the mechanisms modulating meniscal cell proliferation and migration will help to improve approaches for enhancing intrinsic or tissue-engineered repair of the meniscus. The goal of this study was to examine the hypothesis that IL-1 and TNF-a suppress, while TGF-b 1 enhances, cellular proliferation and migration in cell and tissue models of meniscal repair. Methods: A micro-wound assay was used to assess meniscal cell migration and proliferation in response to the following treatments for 0, 24, or 48 hours: 0 to 10 ng/mL IL-1, TNF-a, or TGF-b 1, in the presence or absence of 10% serum. Proliferated and total cells were fluorescently labeled and imaged using confocal laser scanning microscopy and the number of proliferated, migrated, and total cells was determined in the micro-wound and edges of each image. Meniscal cell proliferation was also assessed throughout