monitoring insulin aggregation via capillary electrophoresis通过毛细管电泳监测胰岛素聚合.pdfVIP

Int. J. Mol. Sci. 2011, 12, 9369-9388; doi:10.3390/ijm OPEN ACCESS International Journal of Molecular Sciences ISSN 1422-0067 /journal/ijms Article Monitoring Insulin Aggregation via Capillary Electrophoresis Elizabeth Pryor 1, Joseph A. Kotarek 2,†, Melissa A. Moss 2 and Christa N. Hestekin 1,* 1 Ralph E. Martin Department of Chemical Engineering, 3202 Bell Engineering Center, University of Arkansas, Fayetteville, AR 72701, USA; E-Mail: npryor@ (E.P.) 2 Department of Chemical Engineering, 2C02 Swearingen Engineering Center, University of South Carolina, Columbia, SC 29208, USA; E-Mail: mossme@ † Current address: National Institute of Standards and Technology, 100 Bureau Drive, Stop 8311, Gaithersburg, MD 20899, USA; E-Mail: joseph.kotarek@ . * Author to whom correspondence should be addressed; E-Mail: chesteki@; Tel.: +1-479-575-3416; Fax: +1-479-575-7926. Received: 22 October 2011; in revised form: 6 December 2011 / Accepted: 12 December 2011 / Published: 14 December 2011 Abstract : Early stages of insulin aggregation, which involve the transient formation of oligomeric aggregates, are an important aspect in the progression of Type II diabetes and in the quality control of pharmaceutical insulin production. This study is the first to utilize capillary electrophoresis (CE) with ultraviolet (UV) detection to monitor insulin oligomer formation at pH 8.0 and physiological ionic strength. The la