monitoring immediate-early gene expression through firefly luciferase imaging of hrsj hairless mice监测即早期通过萤火虫荧光素酶基因表达成像hrsj无毛小鼠.pdfVIP

BMC Physiology BioMed Central Methodology article Open Access Monitoring immediate-early gene expression through firefly luciferase imaging of HRS/J hairless mice Anne M Collaco and Michael E Geusz* Address: Department of Biological Sciences and J. P. Scott Center for Neuroscience, Behavior and Mind, 217 Life Science Building, Bowling Green State University, Bowling Green, OH 43403-0212, USA Email: Anne M Collaco - collaa@; Michael E Geusz* - mgeusz@ * Corresponding author Published: 19 August 2003 Received: 19 March 2003 Accepted: 19 August 2003 BMC Physiology 2003, 3:8 This article is available from: /1472-6793/3/8 © 2003 Collaco and Geusz; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the articles original URL. Abstract Background: Gene promoters fused to the firefly luciferase gene (luc) are useful for examining gene regulation in live transgenic mice and they provide unique views of functioning organs. The dynamics of gene expression in cells and tissues expressing luciferase can be observed by imaging this enzymes bioluminescent oxidation of luciferin. Neural pathways involved in specific behaviors have been identified by localizing expression of immediate-early genes such as c-fos. A transgenic mouse line with luc controlled by the human c-fos promoter (fos::luc) has enabled gene expression imaging