monocyte surface expression of fcγ receptor ri (cd64), a biomarker reflecting type-i interferon levels in systemic lupus erythematosus单核细胞表面的表达fcγ受体ri(cd64),一个生物标志物反映系统性红斑狼疮的i型干扰素水平.pdfVIP

Li et al. Arthritis Research Therapy 2010, 12:R90 /content/12/3/R90 R E S E A R C H A R T I C L E Open Access Research article Monocyte surface expression of Fcγ receptor RI (CD64), a biomarker reflecting type-I interferon levels in systemic lupus erythematosus 1 1,2 1 1 1 1 1 1 Yi Li* , Pui Y Lee , Erinn S Kellner , Matthew Paulus , Juliana Switanek , Yuan Xu , Haoyang Zhuang , Eric S Sobel , 2 1 1 Mark S Segal , Minoru Satoh and Westley H Reeves Abstract Introduction: More than half of systemic lupus erythematosus (SLE) patients show evidence of excess type I interferon (IFN-I) production, a phenotype associated with renal disease and certain autoantibodies. However, detection of IFN-I proteins in serum is unreliable, and the measurement of interferon-stimulated gene (ISG) expression is expensive and time consuming. The aim of this study was to identify a surrogate marker for IFN-I activity in clinical samples for monitoring disease activity and response to therapy. Methods: Monocyte surface expression of Fcγ receptors (FcγRs), chemokine receptors, and activation markers were analyzed with flow cytometry in whole blood from patients with SLE and healthy controls. FcγR expression also was measured in peripheral blood mononuclear cells (PBMCs) from healthy controls cultured with Toll-like receptor (TLR) agonists, cytokines, or serum from SLE patients. Expression of ISGs was analyzed with real-time PCR. Results: Circulating CD14+ monocytes from SLE patients showed increased surface expression of FcγRI (CD64). The mean fluorescent intensity of CD64 staining correlated highly with the ISG expression (MX1, IFI44, and Ly6E). In vitro, I