monovalent and unpoised status of most genes in undifferentiated cell-enriched drosophila testis富含单价和unpoised状态大多数基因的未分化的果蝇睾丸.pdfVIP

Gan et al. Genome Biology 2010, 11:R42 /2010/11/4/R42 R E S E A R C H Open Access Research Monovalent and unpoised status of most genes in undifferentiated cell-enriched Drosophila testis 1 2 1 2 2 2 1 Qiang Gan , Dustin E Schones , Suk Ho Eun , Gang Wei , Kairong Cui , Keji Zhao and Xin Chen* tion-associated genes have monovalent, notChromatin in undifferentiated cellsdifferentiation-associatedbivalent histone modifications, in contrast toIn undifferentiated Drosophila cells, differentia- genes in stem cells. Abstract Background: Increasing evidence demonstrates that stem cells maintain their identities by a unique transcription network and chromatin structure. Opposing epigenetic modifications H3K27me3 and H3K4me3 have been proposed to label differentiation-associated genes in stem cells, progenitor and precursor cells. In addition, many differentiation- associated genes are maintained at a poised status by recruitment of the initiative RNA Polymerase II (Pol II) at their promoter regions, in preparation for lineage-specific expression upon differentiation. Previous studies have been performed using cultured mammalian embryonic stem cells. To a lesser extent, chromatin structure has been delineated in other model organisms, such as Drosophila, to open new avenues for genetic analyses. Results: Here we use testes isolated from a Drosophila bag of marbles mutant strain, from which germ cells are in their undifferentiated status. We use these testes to study the endogenous chromatin structure of undifferentiated cells using ChIP-seq. We integrate the ChIP-seq with RNA-seq data, which measures the digital transcriptome. Our genome- wide analyses indicate that most differentiation-associat