multiplex immunoassay platforms based on shape-coded poly(ethylene glycol) hydrogel microparticles incorporating acrylic acid多路复用免疫测定平台基于shape-coded聚(乙二醇)水凝胶微粒将丙烯酸.pdfVIP

Sensors 2012, 12, 8426-8436; doi:10.3390/s120608426 OPEN ACCESS sensors ISSN 1424-8220 /journal/sensors Article Multiplex Immunoassay Platforms Based on Shape-Coded Poly(ethylene glycol) Hydrogel Microparticles Incorporating Acrylic Acid Saemi Park, Hyun Jong Lee and Won-Gun Koh * Department of Chemical and Biomolecular Engineering, Yonsei University, 134 Sinchon-Dong, Seodaemoon-Gu, Seoul 120-749, Korea; E-Mails: saemi.p@ (S.P.); brave520@ (H.J.L.) * Author to whom correspondence should be addressed; E-Mail: wongun@yonsei.ac.kr; Tel.: +82-2-2123-5755; Fax: +82-2-312-6401. Received: 3 May 2012; in revised form: 25 May 2012 / Accepted: 5 June 2012 / Published: 20 June 2012 Abstract: A suspension protein microarray was developed using shape-coded poly(ethylene glycol) (PEG) hydrogel microparticles for potential applications in multiplex and high-throughput immunoassays. A simple photopatterning process produced various shapes of hydrogel micropatterns that were weakly bound to poly(dimethylsiloxane) (PDMS)-coated substrates. These micropatterns were easily detached from substrates during the washing process and were collected as non-spherical microparticles. Acrylic acids were incorporated into hydrogels, which could covalently immobilize proteins onto their surfaces due to the presence of carboxyl groups. The amount of immobilized protein increased with the amount of acrylic acid due to more available carboxyl groups. Saturation