原创力文档multi-species bacterial biofilm and intracellular infection in otitis media中耳炎大型化细菌生物被膜和胞内感染.pdfVIP
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multi-species bacterial biofilm and intracellular infection in otitis media中耳炎大型化细菌生物被膜和胞内感染
Thornton et al. BMC Pediatrics 2011, 11:94
/1471-2431/11/94
RESEARCH ARTICLE Open Access
Multi-species bacterial biofilm and intracellular
infection in otitis media
1,2* 3 1,2 4 2 1,5,6
Ruth B Thornton , Paul J Rigby , Selma P Wiertsema , Pierre Filion , Jennifer Langlands , Harvey L Coates ,
Shyan Vijayasekaran1,5,6, Anthony D Keil7 and Peter C Richmond1,2
Abstract
Background: Bacteria which are metabolically active yet unable to be cultured and eradicated by antibiotic
treatment are present in the middle ear effusion of children with chronic otitis media with effusion (COME) and
recurrent acute otitis media (rAOM). These observations are suggestive of biofilm presence or intracellular
sequestration of bacteria and may play a role in OM pathogenesis. The aim of this project is to provide evidence
for the presence of otopathogenic bacteria intracellularly or within biofilm in the middle ear mucosa of children
with COME or rAOM.
Methods: Middle ear mucosal biopsies from 20 children with COME or rAOM were examined for otopathogenic
bacteria (either in biofilm or located intracellularly) using transmission electron microscopy (TEM) or species specific
fluorescent in situ hybridisation (FISH) and confocal laser scanning microscopy (CLSM). One healthy control biopsy
from a child undergoing cochlear implant surgery was also examined.
Results: No bacteria were observed in the healthy control sample. In 2 of the 3 biopsies imaged using TEM,
bacteria were observed in mucus containing vacuoles within epithelial cells. Bacterial species within these could
not be identified and biofilm was not observed. Using FISH with CLSM, bacteria were seen in 15 of the 17 otitis
med
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