overexpression of the ugt73c6 alters brassinosteroid glucoside formation in arabidopsis thaliana过度的ugt73c6改变brassinosteroid葡萄糖苷形成拟南芥.pdfVIP

Husar et al. BMC Plant Biology 2011, 11:51 /1471-2229/11/51 RESEARCH ARTICLE Open Access Overexpression of the UGT73C6 alters brassinosteroid glucoside formation in Arabidopsis thaliana 1 2 3 1 1 1 4 Sigrid Husar , Franz Berthiller , Shozo Fujioka , Wilfried Rozhon , Mamoona Khan , Florian Kalaivanan , Luisa Elias , 4 4 2 2 3 4 4 Gillian S Higgins , Yi Li , Rainer Schuhmacher , Rudolf Krska , Hideharu Seto , Fabian E Vaistij , Dianna Bowles and Brigitte Poppenberger1,4* Abstract Background: Brassinosteroids (BRs) are signaling molecules that play essential roles in the spatial regulation of plant growth and development. In contrast to other plant hormones BRs act locally, close to the sites of their synthesis, and thus homeostatic mechanisms must operate at the cellular level to equilibrate BR concentrations. Whilst it is recognized that levels of bioactive BRs are likely adjusted by controlling the relative rates of biosynthesis and by catabolism, few factors, which participate in these regulatory events, have as yet been identified. Previously we have shown that the UDP-glycosyltransferase UGT73C5 of Arabidopsis thaliana catalyzes 23-O-glucosylation of BRs and that glucosylation renders BRs inactive. This study identifies the closest homologue of UGT73C5, UGT73C6, as an enzyme that is also able to glucosylate BRs in planta. Results: In a candidate gene approach, in which homologues of UGT73C5 were screened for their potential to induce BR deficiency when over-expressed in plants, UGT73C6 was identified as an enzyme that can glucosylate the BRs CS and BL at their