pathway analysis of the transcriptome and metabolome of salt sensitive and tolerant poplar species reveals evolutionary adaption of stress tolerance mechanisms通路分析转录组和代谢组的盐敏感和宽容揭示杨树物种进化适应压力的耐受机制.pdfVIP

Janz et al. BMC Plant Biology 2010, 10:150 /1471-2229/10/150 RESEARCH ARTICLE Open Access Pathway analysis of the transcriptome and metabolome of salt sensitive and tolerant poplar species reveals evolutionary adaption of stress tolerance mechanisms 1 2 2 3 3 1* Dennis Janz , Katja Behnke , Jörg-Peter Schnitzler , Basem Kanawati , Philippe Schmitt-Kopplin , Andrea Polle Abstract Background: Populus euphratica is a salt tolerant and Populus × canescens a salt sensitive poplar species. Because of low transcriptional responsiveness of P. euphratica to salinity we hypothesized that this species exhibits an innate activation of stress protective genes compared with salt sensitive poplars. To test this hypothesis, the transcriptome and metabolome of mature unstressed leaves of P. euphratica and P. × canescens were compared by whole genome microarray analyses and FT-ICR-MS metabolite profiling. Results: Direct cross-species comparison of the transcriptomes of the two poplar species from phylogenetically different sections required filtering of the data set. Genes assigned to the GO slim categories ‘mitochondria’, ‘cell wall’, ‘transport’, ‘energy metabolism’ and ‘secondary metabolism’ were significantly enriched, whereas genes in the categories ‘nucleus’, ‘RNA or DNA binding’, ‘kinase activity’ and ‘transcription factor activity’ were significantly depleted in P. euphratica compared with P. × canescens. Evidence for a general activation of stress relevant genes in P. euphratica was not detected. Pathway analyses of metabolome and transcriptome data indicated stronger accumulation of primary sugars, activation of pathways for sugar alcohol production, and faster consumption of secondary metabolites in P.