pepgmv rep-protein expression in mammalian cellspepgmv rep蛋白在哺乳动物细胞表达.pdfVIP

Viruses 2012, 4, 1792-1801; doi:10.3390/v4091792 OPEN ACCESS viruses ISSN 1999-4915 /journal/viruses Article PepGMV Rep-Protein Expression in Mammalian Cells Angela María Chapa-Oliver 1, Laura Mejía-Teniente 1, Teresa García-Gasca 2, Ramon Gerardo Guevara-Gonzalez 1 and Irineo Torres-Pacheco 1,* 1 CA Ingeniería de Biosistemas, División de Investigación y Posgrado, Facultad de Ingeniería, Universidad Autónoma de Querétaro, Cerro de las Campanas s/n, Santiago de Querétaro, Qro., 76010, México; E-Mails: angellox02@ (A.M.C.-O.); laurayo@.mx (L.M.-T.); ramon.guevara@uaq.mx (R.G.G.-G.) 2 División de Investigación y Posgrado, Facultad de Ciencias Naturales, Universidad Autónoma de Querétaro, Avenida de las Ciencias s/n, Juriquilla, Qro., 76230, México; E-Mail: tggasca@ * Author to whom correspondence should be addressed; E-Mail: irineo.torres@uaq.mx; torresirineo@; Tel.: +52-442-192-12-52; Fax: +52-442-192-13-12. Received: 27 July 2012; in revisedform: 6 September 2012 / Accepted: 6 September 2012 / Published: 24 September 2012 Abstract: The Geminiviruses genome is a small, single strand DNA that replicates in the plant cell nucleus. Analogous to animal DNA viruses, Geminiviruses depend on the host replication machinery to amplify their genomes and only supply the factors required to initiate their replication. Consequently, Geminiviruses remove the cell-cycle arrest and induce the host replication machinery using an endocycle process. They encode proteins, such as the conserved replica