pet imaging of brain inflammation during early epileptogenesis in a rat model of temporal lobe epilepsypet成像的大脑炎症在早期epileptogenesis颞叶癫痫大鼠模型.pdfVIP

Dedeurwaerdere et al. EJNMMI Research 2012, 2:60 /content/2/1/60 ORIGINAL RESEARCH Open Access PET imaging of brain inflammation during early epileptogenesis in a rat model of temporal lobe epilepsy 1,2* 2 2 2 1 2 Stefanie Dedeurwaerdere , Paul D Callaghan , Tien Pham , Gita L Rahardjo , Halima Amhaoul , Paula Berghofer , 2 2 2 3 2 Mitchell Quinlivan , Filomena Mattner , Christian Loch , Andrew Katsifis and Marie-Claude Grégoire Abstract Background: Recently, inflammatory cascades have been suggested as a target for epilepsy therapy. Positron emission tomography (PET) imaging offers the unique possibility to evaluate brain inflammation longitudinally in a non-invasive translational manner. This study investigated brain inflammation during early epileptogenesis in the post-kainic acid-induced status epilepticus (KASE) model with post-mortem histology and in vivo with [18F]-PBR111 PET. Methods: Status epilepticus (SE) was induced (N = 13) by low-dose injections of KA, while controls (N = 9) received saline. Translocator protein (TSPO) expression and microglia activation were assessed with [125I]-CLINDE autoradiography and OX-42 immunohistochemistry, respectively, 7 days post-SE. In a subgroup of rats, [18F]-PBR111 PET imaging with metabolite-corrected input function was performed before post-mortem evaluation. [18F]-PBR111 volume of distribution ( Vt) in volume of interests (VOIs) was quantified by means of kinetic modelling and a VOI/metabolite-corrected plasma activity ratio. Results: Animals with substantial SE showed huge overexpression