passage and reversal effects on gene expression of bovine meniscal fibrochondrocytes通过和逆转对半月板fibrochondrocytes牛的基因表达的影响.pdfVIP

Available online /content/9/5/R93 Vol 9 No 5 Open Access Research article Passage and reversal effects on gene expression of bovine meniscal fibrochondrocytes Najmuddin J Gunja and Kyriacos A Athanasiou Department of Bioengineering, Rice University, PO Box 1892, Houston, TX 77251, USA Corresponding author: Kyriacos A Athanasiou, athanasiou@ Received: 9 Mar 2007 Revisions requested: 25 Apr 2007 Revisions received: 5 Sep 2007 Accepted: 13 Sep 2007 Published: 13 Sep 2007 Arthritis Research Therapy 2007, 9:R93 (doi:10.1186/ar2293) This article is online at: /content/9/5/R93 © 2007 Gunja and Athanasiou; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract The knee meniscus contains a mixed population of cells that addition, primary and passaged meniscus fibrochondrocytes exhibit fibroblastic as well as chondrocytic characteristics. were placed on surfaces coated with collagen I or aggrecan Tissue engineering studies and future therapies for the protein to investigate whether any gene expression changes meniscus require a large population of cells that are seeded on resulting from passage could be reversed. Collagen I expression scaffolds. To achieve this, monolayer expansion is often used as was found to increase with the number of passages, whereas a technique to increase cell number. However, the phenotype of collagen II and C