phospholipase d2 enhances epidermal growth factor-induced akt activation in el4 lymphoma cells磷脂酶d2增强表皮生长因素akt激活el4淋巴瘤细胞.pdfVIP

Pharmaceuticals 2010, 3, 2045-2058; doi:10.3390/ph3072045 OPEN ACCESS pharmaceuticals ISSN 1424-8247 /journal/pharmaceuticals Article Phospholipase D2 Enhances Epidermal Growth Factor-Induced Akt Activation in EL4 Lymphoma Cells Manpreet S. Chahal 1, Daniel J. Brauner 1 and Kathryn E. Meier 1,2,* 1 Department of Pharmaceutical Sciences, College of Pharmacy, Washington State University, Pullman, Washington, WA 99164-6534, USA; E-Mail: manpreet.chahal@ (M.C.); 2 Program in Nutrition and Exercise Physiology, College of Pharmacy, Washington State University, Spokane, Washington, WA99210-1495, USA * Author to whom correspondence should be addressed; E-Mail: kmeier@ (K.M.); Tel.: +1-509-358-7631; Fax: +1-509-358-7627 Received: 23 April 2010; in revised form: 24 May 2010 / Accepted: 24 June 20010 / Published: 2 July 2010 Abstract: Phospholipase D2 (PLD2) generates phosphatidic acid through hydrolysis of phosphatidylcholine. PLD2 has been shown to play a role in enhancing tumorigenesis. The epidermal growth factor receptor (EGFR) can both activate and interact with PLD2. Murine lymphoma EL4 cells lacking endogenous PLD2 present a unique model to elucidate the role of PLD2 in signal transduction. In the current study, we investigated effects of PLD2 on EGF response. Western blotting and RT-PCR were used to establish that both parental cells and PLD2 transfectants express endogenous EGFR. Levels of EGFR protein are increased in ce