photochemical tissue bonding with chitosan adhesive films光化学组织与壳聚糖胶电影.pdfVIP

Lauto et al. BioMedical Engineering OnLine 2010, 9:47 /content/9/1/47 RESEARCH Open Access Photochemical tissue bonding with chitosan adhesive films 1* 2 1 1 1 3 Antonio Lauto , Damia Mawad , Matthew Barton , Abhishek Gupta , Sabine C Piller , James Hook * Correspondence: a.lauto@uws. Abstract edu.au 1Nanoscale-Organization and Background: Photochemical tissue bonding (PTB) is a promising sutureless Dynamics Group, School of Biomedical and Health Sciences, technique for tissue repair. PTB is often achieved by applying a solution of rose School of Medicine, University of bengal (RB) between two tissue edges, which are irradiated by a green laser to Western Sydney, NSW 1797, crosslink collagen fibers with minimal heat production. In this study, RB has been Australia incorporated in chitosan films to create a novel tissue adhesive that is laser-activated. Methods: Adhesive films, based on chitosan and containing ~0.1 wt% RB were manufactured and bonded to calf intestine by a solid state laser (l = 532 nm, Fluence~110 J/cm2, spot size~0.5 cm). A single-column tensiometer, interfaced with a personal computer, tested the bonding strength. K-type thermocouples recorded the temperature (T) at the adhesive-tissue interface during laser irradiation. Human fibroblasts were also seeded on the adhesive and cultured for 48 hours to assess cell growth.