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phytoecdysteroids from the roots of achyranthes bidentata blumephytoecdysteroids牛膝根的bidentata抱蛋.pdf

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phytoecdysteroids from the roots of achyranthes bidentata blumephytoecdysteroids牛膝根的bidentata抱蛋

Molecules 2012, 17, 3324-3332; doi:10.3390/molecule OPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article Phytoecdysteroids from the Roots of Achyranthes bidentata Blume Mei Zhang 1,2, Zhong-Yu Zhou 1, Jing Wang 1,2, Yong Cao 3, Xue-Xiang Chen 3, Wei-Min Zhang 4, Li-Dong Lin 1 and Jian-Wen Tan 1,* 1 Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China 2 Graduate School of the Chinese Academy of Sciences, Beijing 100039, China 3 College of Food Science, South China Agricultural University, Guangzhou 510642, China 4 Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Institute of Microbiology, Guangzhou 510070, China * Author to whom correspondence should be addressed; E-Mail: jwtan@; Tel.: +86-20-3708-2054; Fax: +86-20-3708-2054. Received: 23 February 2012; in revised form: 8 March 2012 / Accepted: 12 March 2012 / Published: 14 March 2012 Abstract: Two new phytoecdysteroids, (25S)-20,22-O-(R-ethylidene)inokosterone (1) and 20,22-O-(R-3-methoxycarbonyl)propylidene-20-hydroxyecdysone (2), together with six known phytoecdysteroids 3–8 were isolated from the roots of Achyranthes bidentata Blume. The new structures were established on the basis of spectroscopic studies and chemical evidences. The absolute configuration at C-25 in the structure of known compound 3 was determined by chemical and spectroscopic means.