piezoelectric sensor for determination of genetically modified soybean roundup readya in samples not amplified by pcr压电传感器测定转基因大豆综述readya pcr在样本不放大.pdfVIP

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piezoelectric sensor for determination of genetically modified soybean roundup readya in samples not amplified by pcr压电传感器测定转基因大豆综述readya pcr在样本不放大.pdf

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piezoelectric sensor for determination of genetically modified soybean roundup readya in samples not amplified by pcr压电传感器测定转基因大豆综述readya pcr在样本不放大

Sensors 2007, 7, 1462-1479 sensors ISSN 1424-8220 © 2007 by MDPI /sensors Full Research Paper Piezoelectric Sensor for Determination of Genetically Modified   Soybean Roundup Ready in Samples not Amplified by PCR Magdalena Stobiecka 1, Jarosław M. Cieśla 2, Beata Janowska 2, Barbara Tudek 2, 3 and Hanna Radecka 1,* 1 Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10, 10-474 OLSZTYN, POLAND E-mail: stobieckam@pan.olsztyn.pl; E-mail: hanna.radecka@pan.olsztyn.pl 2 Genetic Modifications Analysis Laboratory, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawińskiego 5a, 02-106 WARSAW, POLAND E-mail: castor@ibb.waw.pl; E-mail: r_b@ibb.waw.pl; E-mail: tudek@ibb.waw.pl 3 Institute of Genetics and Biotechnology, Warsaw University, Pawińskiego 5a, 02-106 WARSAW, POLAND * Author to whom correspondence should be addressed . E-mail: hanna.radecka@pan.olsztyn.pl; Tel.: +48 89 523 4636 Received: 19 July 2007/ Accepted: 10 August 2007 / Published: 14 August 2007 Abstract: The chemically modified piezoelectrodes were utilized to develop relatively cheap and easy to use biosensor for determination of genetically modified Roundup Ready soybean (RR soybean). The biosensor relies on the immobilization onto gold piezoelectrodes of the 21-mer single stranded oligonucleotide (probes) related to 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) gene, which is an