post-operative infection and sepsis in humans is associated with deficient gene expression of γc cytokines and their apoptosis mediators术后感染和脓毒症在人类与γc细胞因子的基因表达和细胞凋亡不足介质.pdfVIP

White et al. Critical Care 2011, 15:R158 /content/15/3/R158 RESEARCH Open Access Post-operative infection and sepsis in humans is associated with deficient gene expression of gc cytokines and their apoptosis mediators 1,2* 3 1 4 5 2 Mary White , Vivienne Mahon , Robert Grealy , Derek G Doherty , Patrick Stordeur , Dermot P Kelleher , Ross McManus2 and Thomas Ryan1 Abstract Introduction: Lymphocyte homeostasis is dependent on the gc cytokines. We hypothesised that sepsis in humans is associated with differential gene expression of the gc cytokines and their associated apoptosis mediators. Methods: The study population consisted of a total of 60 patients with severe sepsis, 15 with gram negative bacteraemia, 10 healthy controls and 60 patients undergoing elective lung resection surgery. Pneumonia was diagnosed by CDC NNIC criteria. Gene expression in peripheral blood leukocytes (PBLs) of interleukin (IL)-2, 7, 15 and interferon (IFN)-g, Bax, Bim, Bcl-2 was determined by qRT-PCR and IL-2 and IL-7 serum protein levels by ELISA. Gene expression of IL-2, 7 and IFN-g was measured in peripheral blood leukocytes (PBL), cultured in the presence of lipopolysacharide (LPS) and CD3 binding antibody (CD3ab) Results: IL-2 gene expression was lower in the bacteraemia group compared with controls, and lower still in the sepsis group (P 0.0001). IL-7 gene expression was similar in controls and bacteraemia, but lower in sepsis (P 0.0001). IL-15 gene expression was similar in the three groups. Bcl-2 gene expression was less (P 0.0001) and Bim gene expression was greater (P = 0.0003) in severe sepsis compared to bacteraemic and healthy controls. Bax gene expression was similar in the three groups.