profiling of phenolic compounds and antioxidant activity of dry extracts from the selected sorbus species分析酚类化合物和干提取物的抗氧化活性所选花楸属的物种.pdfVIP

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profiling of phenolic compounds and antioxidant activity of dry extracts from the selected sorbus species分析酚类化合物和干提取物的抗氧化活性所选花楸属的物种.pdf

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profiling of phenolic compounds and antioxidant activity of dry extracts from the selected sorbus species分析酚类化合物和干提取物的抗氧化活性所选花楸属的物种

Molecules 2012, 17, 3093-3113; doi:10.3390/molecule OPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article Profiling of Phenolic Compounds and Antioxidant Activity of Dry Extracts from the Selected Sorbus Species Monika A. Olszewska *, Anna Presler and Piotr Michel Department of Pharmacognosy, Faculty of Pharmacy, Medical University of Lodz, Muszyńskiego 1, 90-151 Lodz, Poland; E-Mails: anpre@wp.pl (A.P.); piotr.michel@umed.lodz.pl (P.M.) * Author to whom correspondence should be addressed; E-Mail: monika.olszewska@umed.lodz.pl; Tel.: +48-42-677-9169; Fax: +48-42-678-8398. Received: 15 February 2012; in revised form: 5 March 2012 / Accepted: 6 March 2012 / Published: 12 March 2012 Abstract: The antioxidant efficiency of dry extracts from inflorescences and/or leaves of seven Sorbus species was studied using four in vitro tests of SET (single electron transfer) and HAT-type (hydrogen atom transfer) mechanisms. The 70% methanol extracts and its diethyl ether, ethyl acetate, n-butanol and water fractions were tested in parallel with the phenolic standards, e.g., caffeic acid, quercetin, BHA, BHT, and Trolox. The SET-type activity of the extracts depended primarily on the extraction solvent. The most valuable extracts were n-butanol and ethyl acetate ones, which activity was high in the DPPH ® (EC50 = 3.2–5.2 μg/mL), TEAC (2.8–4.0 mmol Trolox /g), and FRAP (9.8–13.7 mmol