pretargeted immuno-pet of cea-expressing intraperitoneal human colonic tumor xenografts a new sensitive detection methodpretargeted immuno-pet cea-expressing的腹腔内人类结肠肿瘤异种移植新的敏感的检测方法.pdfVIP

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pretargeted immuno-pet of cea-expressing intraperitoneal human colonic tumor xenografts a new sensitive detection methodpretargeted immuno-pet cea-expressing的腹腔内人类结肠肿瘤异种移植新的敏感的检测方法.pdf

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pretargeted immuno-pet of cea-expressing intraperitoneal human colonic tumor xenografts a new sensitive detection methodpretargeted immuno-pet cea-expressing的腹腔内人类结肠肿瘤异种移植新的敏感的检测方法

Schoffelen et al. EJNMMI Research 2012, 2:5 /content/2/1/5 ORIGINAL RESEARCH Open Access Pretargeted immuno-PET of CEA-expressing intraperitoneal human colonic tumor xenografts: a new sensitive detection method 1* 2 3 1 4 Rafke Schoffelen , Winette TA van der Graaf , Robert M Sharkey , Gerben M Franssen , William J McBride , 5 1 3 1 1 Chien-Hsing Chang , Peter Laverman , David M Goldenberg , Wim JG Oyen and Otto C Boerman Abstract Background: In this study, pretargeted immuno-positron-emission tomography [PET] with a bispecific monoclonal anti-carcinoembryonic antigen [CEA] (CEACAM5) × anti-hapten antibody (bispecific monoclonal antibody [bsmAb]) and a small (1.5 kD) peptide labeled with 68Ga was compared to fludeoxyglucose [18F-FDG]-PET for detecting intraperitoneal [i.p.] CEA-expressing human colonic tumor xenografts in nude mice. Methods: Two groups of female BALB/c nude mice were inoculated with LS174T human colonic tumor cells i.p. One group received 5 MBq 18F-FDG, and the other received intravenous injections of the bsmAb, followed 16 h later with 5 MBq of 68Ga-labeled peptide. One hour after the radiolabeled peptide or FDG was given, micro-PET/ computed tomography images were acquired. Thereafter, the uptake of the 68Ga or 18F in dissected tissue was determined. Results: Within 1 h, high uptake of the 68Ga-labeled peptide in the tumor lesions (23.4 ± 7.2% ID/g) and low background activity levels were observed (e.g., tumor-to-intestine ratio, 58 ± 22). This resulted in a clear visualization of all intra-abdominal tumor lesions ≥ 10 μL and even some tumo