原创力文档pressurized liquid extraction of coumarins from fruits of heracleum leskowii with application of solvents with different polarity under increasing temperature加压液相萃取水果的香豆素heracleum leskowii与不同极性溶剂的应用受到越来越多的温度.pdfVIP
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pressurized liquid extraction of coumarins from fruits of heracleum leskowii with application of solvents with different polarity under increasing temperature加压液相萃取水果的香豆素heracleum leskowii与不同极性溶剂的应用受到越来越多的温度
Molecules 2012, 17, 4133-4141; doi:10.3390/molecule
OPEN ACCESS
molecules
ISSN 1420-3049
/journal/molecules
Article
Pressurized Liquid Extraction of Coumarins from Fruits of
Heracleum leskowii with Application of Solvents with Different
Polarity under Increasing Temperature
Krystyna Skalicka-Woźniak * and Kazimierz Głowniak
Department of Pharmacognosy with Medicinal Plant Unit, Medical University in Lublin, 1 Chodzki,
Lublin 20-093, Poland; E-Mail: kglowniak@
* Author to whom correspondence should be addressed; E-Mail: kskalicka@;
Tel.: +48-81-742-3807; Fax: +48-81-742-3809.
Received: 11 March 2012; in revised form: 22 March 2012 / Accepted: 31 March 2012 /
Published: 5 April 2012
Abstract: Coumarins are nowadays an important group of organic compounds from
natural sources that are useful in a number of fields. Because they possess different
pharmacological properties, finding the proper extraction conditions for their separation
from plant matrices is a very important step. In this report Pressurized Liquid Extraction
(PLE) under different temperature conditions and with different types of extraction
solvents were tested. As a matrix, fruits of Heracleum leskowii have been used. A simple
reverse phase high-performance liquid chromatographic method (RP-HPLC) coupled with
a photodiode array detector (DAD) has been developed for separation and quantitative
analysis of the main coumarins. Umbelliferone, xanthot
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