predicting the sensitivity and specificity of published real-time pcr assays预测发布实时pcr检测的敏感性和特异性.pdf

Annals of Clinical Microbiology and Antimicrobials BioMed Central Research Open Access Predicting the sensitivity and specificity of published real-time PCR assays 1 2 Gordon H Lemmon and Shea N Gardner* Address: 1Center for Structural Biology, Vanderbilt University, 465 21st Ave. South, BIOSCI/MRB III suite 5140, Nashville, TN 37240, USA and 2Pathogen Bioinformatics, Lawrence Livermore National Laboratory, PO Box 808, L-174, Livermore, CA 94551, USA Email: Gordon H Lemmon - gordon.lemmon@; Shea N Gardner* - gardner26@LLNL.gov * Corresponding author Published: 25 September 2008 Received: 17 June 2008 Accepted: 25 September 2008 Annals of Clinical Microbiology and Antimicrobials 2008, 7:18 doi:10.1186/1476-0711-7-18 This article is available from: /content/7/1/18 © 2008 Lemmon and Gardner; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Background: In recent years real-time PCR has become a leading technique for nucleic acid detection and quantification. These assays have the potential to greatly enhance efficiency in the clinical laboratory. Choice of primer and probe sequences is critical for accurate diagnosis in the clinic, yet current pri