protein turnover in mycobacterial proteomics在分枝杆菌蛋白质组学蛋白质周转.pdfVIP

Molecules 2009, 14, 3237-3258; doi:10.3390/molecule OPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Review Protein Turnover in Mycobacterial Proteomics Prahlad K. Rao 1,* and Qingbo Li 1,2,* 1 Center for Pharmaceutical Biotechnology, College of Pharmacy, University of Illinois at Chicago, Chicago, Illinois 60607, USA 2 Department of Microbiology and Immunology, College of Medicine, University of Illinois at Chicago, Chicago, Illinois 60612, USA * Authors to whom correspondence should be addressed; E-mails: rkrish4@ (P-K.R.); qkli@ (Q.L.). Received: 1 July 2009; in revised form: 21 August 2009 / Accepted: 26 August 2009 / Published: 28 August 2009 Abstract: Understanding the biology of Mycobacterium tuberculosis is one of the primary challenges in current tuberculosis research. Investigation of mycobacterial biology using the systems biology approach has deciphered much information with regard to the bacilli and tuberculosis pathogenesis. The modulation of its environment and the ability to enter a dormant phase are the hallmarks of M. tuberculosis . Until now, proteome studies have been able to understand much about the role of various proteins, mostly in growing M. tuberculosis cells. It has been difficult to study dormant M. tuberculosis by conventional proteomic techniques with very few proteins being found to be differentially expressed. Discrepancy between proteome and transcriptome