proteomic identification of oscyp2, a rice cyclophilin that confers salt tolerance in rice (oryza sativa l.) seedlings when overexpressed蛋白质组学鉴定oscyp2还有米饭,授予对(栽培稻l .)苗水稻耐盐中.pdfVIP

Ruan et al. BMC Plant Biology 2011, 11:34 /1471-2229/11/34 RESEARCH ARTICLE Open Access Proteomic identification of OsCYP2, a rice cyclophilin that confers salt tolerance in rice (Oryza sativa L.) seedlings when overexpressed 1,2* 1* 1 2 1 2 1 Song-Lin Ruan , Hua-Sheng Ma , Shi-Heng Wang , Ya-Ping Fu , Ya Xin , Wen-Zhen Liu , Fang Wang , 1 1 2 Jian-Xin Tong , Shu-Zhen Wang , Hui-Zhe Chen Abstract Background: High Salinity is a major environmental stress influencing growth and development of rice. Comparative proteomic analysis of hybrid rice shoot proteins from Shanyou 10 seedlings, a salt-tolerant hybrid variety, and Liangyoupeijiu seedlings, a salt-sensitive hybrid variety, was performed to identify new components involved in salt-stress signaling. Results: Phenotypic analysis of one protein that was upregulated during salt-induced stress, cyclophilin 2 (OsCYP2), indicated that OsCYP2 transgenic rice seedlings had better tolerance to salt stress than did wild-type seedlings. Interestingly, wild-type seedlings exhibited a marked reduction in maximal photochemical efficiency under salt stress, whereas no such change was observed for OsCYP2-transgenic seedlings. OsCYP2-transgenic seedlings had lower levels of lipid peroxidation products and higher activities of antioxidant enzymes than wild-type seedlings. Spatiotemporal expression analysis of OsCYP2 showed that it could be induced by salt stress in both Shanyou 10 and Liangyoupeijiu seedlings, but Shanyou 10 seedlings showed higher OsCYP2 expression levels. Moreover, circadian rhythm expression of OsCYP2 in Shanyou 10 seedlings occurred earlier than in Liangyoupeijiu seedlings. Treatmen