proteomic characterization of iron deficiency responses in cucumis sativus l. roots蛋白质组学鉴定的缺铁反应cucumis巨大成功的根源.pdfVIP

Donnini et al. BMC Plant Biology 2010, 10:268 /1471-2229/10/268 RESEARCH ARTICLE Open Access Proteomic characterization of iron deficiency responses in Cucumis sativus L. roots * Silvia Donnini, Bhakti Prinsi, Alfredo S Negri, Gianpiero Vigani, Luca Espen, Graziano Zocchi Abstract Background: Iron deficiency induces in Strategy I plants physiological, biochemical and molecular modifications capable to increase iron uptake from the rhizosphere. This effort needs a reorganization of metabolic pathways to efficiently sustain activities linked to the acquisition of iron; in fact, carbohydrates and the energetic metabolism has been shown to be involved in these responses. The aim of this work was to find both a confirmation of the already expected change in the enzyme concentrations induced in cucumber root tissue in response to iron deficiency as well as to find new insights on the involvement of other pathways. Results: The proteome pattern of soluble cytosolic proteins extracted from roots was obtained by 2-DE. Of about two thousand spots found, only those showing at least a two-fold increase or decrease in the concentration were considered for subsequent identification by mass spectrometry. Fifty-seven proteins showed significant changes, and 44 of them were identified. Twenty-one of them were increased in quantity, whereas 23 were decreased in quantity. Most of the increased proteins belong to glycolysis and nitrogen metabolism in agreement with the biochemical evidence. On the other hand, the proteins being decreased belong to the metabolism of sucrose and complex structural carbohydrates and to structural proteins. Conclusions: The new available techniques allow to cast new light on the mechanisms