pseudomonas fluorescens hk44 lessons learned from a model whole-cell bioreporter with a broad application history荧光假hk44教训一个模型全细胞bioreporter有着广阔的应用历史.pdfVIP

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pseudomonas fluorescens hk44 lessons learned from a model whole-cell bioreporter with a broad application history荧光假hk44教训一个模型全细胞bioreporter有着广阔的应用历史.pdf

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pseudomonas fluorescens hk44 lessons learned from a model whole-cell bioreporter with a broad application history荧光假hk44教训一个模型全细胞bioreporter有着广阔的应用历史

Sensors 2012, 12, 1544-1571; doi:10.3390/s120201544 OPEN ACCESS sensors ISSN 1424-8220 /journal/sensors Review Pseudomonas fluorescens HK44: Lessons Learned from a Model Whole-Cell Bioreporter with a Broad Application History Josef Trögl 1,*, Archana Chauhan 2, Steven Ripp 2, Alice C. Layton 2, Gabriela Kuncová3 and 2 Gary S. Sayler 1 Faculty of the Environment, Jan Evangelista Purkyně University in Ústí nad Labem, Králova Výšina 3132/7, 400 96 Ústí nad Labem, Czech Republic 2 The Center for Environmental Biotechnology, The University of Tennessee, 676 Dabney Hall, Knoxville, TN 37996, USA; E-Mails: achauha1@ (A.C.); saripp@ (S.R.); alayton@ (A.C.L.); sayler@ (G.S.S.) 3 Institute of Chemical Process Fundamentals of ASCR, v.v.i., Rozvojová135, 165 02, Prague 6-Suchdol, Czech Republic; E-Mail: kuncova@icpf.cas.cz * Author to whom correspondence should be addressed; E-Mail: josef.trogl@ujep.cz; Tel.: +420-475-284-151; Fax: +420-475-284-158. Received: 29 December 2011; in revised form: 24 January 2012 / Accepted: 3 February 2012 / Published: 6 February 2012 Abstract: Initially described in 1990, Pseudomonas fluorescens HK44 served as the first whole-cell bioreporter genetically endowed with a bioluminescent (luxCDABE) phenotype directly linked to a catabolic (naphthalene degradative) pathway. HK44 was the first genetically engineered microorganism to be released in the field to monitor bioremediation potential. Subsequent to that release, strain HK44 had been introduced