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radical scavenging and reducing power of salvia mirzayanii subfractions自由基清除和减少的鼠尾草mirzayanii小分支.pdf

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radical scavenging and reducing power of salvia mirzayanii subfractions自由基清除和减少的鼠尾草mirzayanii小分支

Molecules 2008, 13, 2804-2813; DOI: 10.3390/ molecule OPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Communication Radical Scavenging and Reducing Power of Salvia mirzayanii Subfractions Mahmood Reza Moein 1, Soheila Moein 2, * and Saeid Ahmadizadeh 2 1 Department of Pharmacognosy and Pharmaceutical Sciences Research center, Faculty of Pharmacy, Shiraz University of Medical Sciences, Karafarin St., Shiraz 71345-1583, Iran; E-mail: mrmoein@sums.ac.ir, mrezamoein@ 2 Biochemistry Department, Faculty of Medicine, and Infectious Disease Research Center, Hormozgan University of Medical Sciences, Shaheed Naser Blvd., Bandar Abbas 79149-64153, Iran. * Author to whom correspondence should be addressed; E-mail: smoein@razi.tums.ac.ir or soheila_9@; Tel: +987613332424; Fax: +987613330612. Received: 29 June 2008; in revised form: 19 September 2008/ Accepted: 3 November 2008 / Published: 12 November 2008 Abstract: In this research, the radical scavenging activity and reducing power of the ethyl acetate fraction and subfractions of Salvia mirzayanii (SM) have been investigated. The plant material was initially extracted with ethanol. The fractionation was carried out using liquid-liquid extraction, then the ethyl acetate fraction, which showed the greatest antioxidant activity, was selected. This fraction was submitted to column chromatography on a Sephadex LH 20 column eluted with pure MeOH to obtain subfractions A-G. No significant differences exist b