proteome characterization of developing grains in bread wheat cultivars (triticum aestivum l.)蛋白质组表征的发展中谷物面包小麦品种(小麦l .).pdfVIP

Guo et al. BMC Plant Biology 2012, 12:147 /1471-2229/12/147 RESEARCH ARTICLE Open Access Proteome characterization of developing grains in bread wheat cultivars (Triticum aestivum L.) † † † Guangfang Guo , Dongwen Lv , Xing Yan , Saminathan Subburaj, Pei Ge, Xiaohui Li, Yingkao Hu * and Yueming Yan Abstract Background: The analyses of protein synthesis, accumulation and regulation during grain development in wheat are more complex because of its larger genome size compared to model plants such as Arabidopsis and rice. In this study, grains from two wheat cultivars Jimai 20 and Zhoumai 16 with different gluten quality properties were harvested at five development stages, and were used to displayed variable expression patterns of grain proteins. Results: Proteome characterization during grain development in Chinese bread wheat cultivars Jimai 20 and Zhoumai 16 with different quality properties was investigated by 2-DE and tandem MALDI-TOF/TOF-MS. Identification of 117 differentially accumulated protein spots representing 82 unique proteins and five main expression patterns enabled a chronological description of wheat grain formation. Significant proteome expression differences between the two cultivars were found; these included 14 protein spots that accumulated in both cultivars but with different patterns and 27 cultivar-different spots. Among the cultivar-different protein spots, 14 accumulated in higher abundance in Jimai 20 than in Zhoumai 16, and included NAD-dependent isocitrate dehydrogenase, triticin precursor, LMW-s glutenin subunit and replication factor C-like protein. These proteins are likely to be associated with superior gluten quality. In addition, some proteins such as class II chitinase and peroxidase 1 with isoforms in d