radiation-induced dna damage and repair in human γδ and αβ t-lymphocytes analysed by the alkaline comet assay辐射诱导的dna损伤和修复人类γδαβt淋巴球碱性彗星试验分析.pdfVIP

Lisowska et al. Genome Integrity 2010, 1:8 /content/1/1/8 GENOME INTEGRITY RESEARCH Open Access Radiation-induced DNA damage and repair in human gδ and ab T-lymphocytes analysed by the alkaline comet assay 1 1,2 3 4 1,3* Halina Lisowska , Marta Deperas-Kaminska , Siamak Haghdoost , Ingela Parmryd , Andrzej Wojcik Abstract It has been shown by a number of authors that the radiosensitivity of peripheral blood mononuclear cells (PBMC) is higher in cancer patients compared to healthy donors, which is interpreted as a sign of genomic instability. PBMC are composed of different cell subpopulations which are differently radiosensitive and the difference between cancer patients and healthy donors could also be due to different composition of their PBMC pools. Gamma-delta T-lymphocytes play an important role in immunosurveillance and are promising cells for immuno- therapy. Their abundance is frequently reduced in cancer patients so should their sensitivity to radiation be lower than that of other T-lymphocytes, this could, at least partly explain the low radiosensitivity of PBMC from healthy individuals compared to cancer patients. The present investigation was carried out to test this. Using the alkaline comet assay we analysed the level of DNA damage and repair in isolated gδ T-lymphocytes, pan T-lymphocytes and in total PBMC exposed in vitro to gamma radiation. We found no difference in the level of DNA damage and the capacity of DNA repair between the T cell populations. This is the first study that addresses the question of sensitivity to radiation of gamma-delta T-cells. Background