原创力文档rapid detection of pseudomonas aeruginosa from positive blood cultures by quantitative pcr铜绿假单胞菌的快速检测定量pcr阳性血培养.pdfVIP
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rapid detection of pseudomonas aeruginosa from positive blood cultures by quantitative pcr铜绿假单胞菌的快速检测定量pcr阳性血培养
Cattoir et al. Annals of Clinical Microbiology and Antimicrobials 2010, 9:21
/content/9/1/21
RESEARCH Open Access
Rapid detection of Pseudomonas aeruginosa from
positive blood cultures by quantitative PCR
1,2* 1 1 1 1
Vincent Cattoir , Audrey Gilibert , Jeanne-Marie Le Glaunec , Nathalie Launay , Lilia Bait-Mérabet ,
Patrick Legrand1
Abstract
Background: Pseudomonas aeruginosa is responsible for numerous bloodstream infections associated with severe
adverse outcomes in case of inappropriate initial antimicrobial therapy. The present study was aimed to develop a
novel quantitative PCR (qPCR) assay, using ecfX as the specific target gene, for the rapid and accurate identification
of P. aeruginosa from positive blood cultures (BCs).
Methods: Over the period August 2008 to June 2009, 100 BC bottles positive for gram-negative bacilli were tested
in order to evaluate performances of the qPCR technique with conventional methods as gold standard (i.e. culture
and phenotypic identification).
Results: Thirty-three strains of P. aeruginosa, 53 strains of Enterobactericaeae, nine strains of Stenotrophomonas
maltophilia and two other gram-negative species were isolated while 3 BCs were polymicrobial including one
mixture containing P. aeruginosa. All P. aeruginosa clinical isolates were detected by qPCR except a single strain in
mixed culture. Performances of the qPCR technique were: specificity, 100%; positive predictive value, 100%;
negative predictive value, 98.5%; and sensitivity, 97%.
Conclusions: This reliable technique may offer a rapid (1.5 h) tool that would help clinicians to initiate an
appropriate treatment earlier. Further investigations are needed to assess the clinical benefit of t
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