real time microelectrode measurement of nitric oxide in kidney tubular fluid in vivo实时微电极测量肾小管液在体内的一氧化氮.pdfVIP

Sensors 2003, 3, 314-320 sensors ISSN 1424-8220 © 2003 by MDPI /sensors Real Time Microelectrode Measurement of Nitric Oxide in Kidney Tubular Fluid in vivo * David Z. Levine and Michelle Iacovitti Division of Nephrology, The Kidney Research Centre, Ottawa Health Research Institute and University of Ottawa, 451 Smyth Road, Room 1333, Ottawa, Ontario, CANADA K1H 8M5, Tel: (613) 562-5800, ext 8240, Fax: (613) 562-5487 *Author to whom correspondence should be addressed. E-mail: dzlevine @uottawa.ca Received: 06 December 2003 / Accep ted: 22 January 2003 / Published: 22 A ugust 2003 Abstract: In this review we summarize our experience using a microelectrode to measure nitric oxide concentrations [NO] in living rat kidney tubules. In the anaesthetized living rat, the abdomen can be opened, and the kidney can be placed in a cup such that one can puncture a surface single tubular segment, 1-2 mm long, connected to one of 30,000 filtering glomeruli. The tubular segment can be viewed with a stereo microscope and punctured using sophisticated micromanipulators. The segment, ranging in diameter from about 15 - 35 um contains freely flowing RBC-free fluid, electrolytes, O , pCO and NO gas 2 2 concentrations, and a host of other known and unknown substances. After a “pre” puncture with a 7-10 um beveled glass pipette, intratubular