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real-time monitoring of non-specific toxicity using a saccharomyces cerevisiae reporter system实时监控系统使用酿酒酵母的非特异性毒性记者.pdf

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real-time monitoring of non-specific toxicity using a saccharomyces cerevisiae reporter system实时监控系统使用酿酒酵母的非特异性毒性记者

Sensors 2008, 8, 6433-6447; DOI: 10.3390/s8106433 OPEN ACCESS sensors ISSN 1424-8220 /sensors Article Real-time Monitoring of Non-specific Toxicity Using a Saccharomyces cerevisiae Reporter System Anna-Liisa Välimaa 1,*, Anniina Kivistö 1, Marko Virta 3 and Matti Karp 1,2 1 Department of Chemistry and Bioengineering and 2 Biosensing Competence Centre (BCC), Tampere University of Technology, P.O. Box 541, FI- 33101 Tampere, Finland; E-Mails: anniina.kivisto@tut.fi (A.K.); matti.karp@tut.fi (M.K.) 3 Department of Applied Chemistry and Microbiology Division of Microbiology, P.O. Box 56, FI-00014, University of Helsinki, Finland; E-Mails: marko.virta@helsinki.fi (M.V.) * Author to whom correspondence should be addressed; E-Mails: anna-liisa.valimaa@tut.fi (A.L); Tel.: +358-3-3115-2968; Fax: +358-3-3115-2869 Received: 31 August 2008; in revised form: 26 September 2008 / Accepted: 7 October 2008 / Published: 16 October 2008 Abstract: Baker’s yeast, Saccharomyces cerevisiae, is the simplest and most well-known representative of eukaryotic cells and thus a convenient model organism for evaluating toxic effects in human cells and tissues. Yeast cell sensors are easy to maintain with short generation times, which makes the analytical method of assessing antifungal toxicity cheap and less-time consuming. In this work, the toxicity of test compounds was assessed in bioassays based on bioluminescence inhibition and on traditional growth inhibition on agar plates. The model org