pulsatile ex vivo perfusion of human saphenous vein grafts under controlled pressure conditions increases mmp-2 expression跳动的人类隐静脉移植体外灌注控制压力条件下增加mmp-2表达式.pdfVIP
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pulsatile ex vivo perfusion of human saphenous vein grafts under controlled pressure conditions increases mmp-2 expression跳动的人类隐静脉移植体外灌注控制压力条件下增加mmp-2表达式
Dummler et al. BioMedical Engineering OnLine 2011, 10:62
/content/10/1/62
RESEARCH Open Access
Pulsatile ex vivo perfusion of human saphenous
vein grafts under controlled pressure conditions
increases MMP-2 expression
1*† 1*† 1 1 1 1
Sara Dummler , Stefan Eichhorn , Christian Tesche , Ulrich Schreiber , Bernhard Voss , Marcus-André Deutsch ,
2 1 1 1
Hans Hauner , Harald Lahm , Rüdiger Lange and Markus Krane
* Correspondence: dummler@dhm. Abstract
mhn.de; stefan.eichhorn@dhm.
mhn.de Background: The use of human saphenous vein grafts (HSVGs) as a bypass conduit
1German Heart Center Munich at
the Technische Universität is a standard procedure in the treatment of coronary artery disease while their early
München, Department of occlusion remains a major problem.
Cardiovascular Surgery,
Lazarettstrasse 36, D-80636 Munich, Methods: We have developed an ex vivo perfusion system, which uses standardized
Germany and strictly controlled hemodynamic parameters for the pulsatile and non-static
Full list of author information is perfusion of HSVGs to guarantee a reliable analysis of molecular parameters under
available at the end of the article
different pressure conditions. Cell viability of HSVGs (n = 12) was determined by the
metabolic conversion of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide
(MTT) into a purple formazan dye.
Results: Under physiological
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