prokaryotic aminopeptidase activity along a continuous salinity gradient in a hypersaline coastal lagoon (the coorong, south australia)原核的氨肽酶活动沿着一个连续的盐度梯度沿海咸水泻湖(coorong,南澳大利亚).pdfVIP

Pollet et al. Saline Systems 2010, 6:5 /content/6/1/5 SALINE SYSTEMS S HO RT REPORT Open Access Short Report Prokaryotic aminopeptidase activity along a continuous salinity gradient in a hypersaline coastal lagoon (the Coorong, South Australia) 1,3 1 1,4 1 Thomas Pollet*1,2, Mathilde Schapira , Marie-Jeanne Buscot , Sophie C Leterme , James G Mitchell and Laurent Seuront1,4,5,6 Abstract The distribution and aminopeptidase activity of prokaryotes were investigated along a natural continuous salinity gradient in a hypersaline coastal lagoon, the Coorong, South Australia. The abundance of prokaryotes significantly increased from brackish to hypersaline waters and different sub-populations, defined by flow cytometry, were observed along the salinity gradient. While four sub-populations were found at each station, three additional ones were observed for 8.3% and 13.4%, suggesting a potential modification in the composition of the prokaryotic communities and/or a variation of their activity level along the salinity gradient. The aminopeptidase activity highly increased along the gradient and salinity appeared as the main factor favouring this enzymatic activity. However, while the aminopeptidase activity was dominated by free enzymes for salinities ranging from 2.6% to 13.4%, cell-attached aminopeptidase activity was predominant in more saline waters (i.e. 15.4%). Changes in substrate structure and availability, strongly related to salinity, might (i) modify patterns of both aminopeptidase activities (free and cell- associated enzymes) and (ii) obligate the prokaryotic communitie