resveratrol mediated modulation of sirt-1runx2 promotes osteogenic differentiation of mesenchymal stem cells potential role of runx2 deacetylation白藜芦醇介导调制sirt-1runx2促进成骨分化的间充质干细胞的潜在作用runx2脱乙酰作用.pdfVIP

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resveratrol mediated modulation of sirt-1runx2 promotes osteogenic differentiation of mesenchymal stem cells potential role of runx2 deacetylation白藜芦醇介导调制sirt-1runx2促进成骨分化的间充质干细胞的潜在作用runx2脱乙酰作用.pdf

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resveratrol mediated modulation of sirt-1runx2 promotes osteogenic differentiation of mesenchymal stem cells potential role of runx2 deacetylation白藜芦醇介导调制sirt-1runx2促进成骨分化的间充质干细胞的潜在作用runx2脱乙酰作用

Resveratrol Mediated Modulation of Sirt-1/Runx2 Promotes Osteogenic Differentiation of Mesenchymal Stem Cells: Potential Role of Runx2 Deacetylation 1 2 1 1 1 Mehdi Shakibaei *, Parviz Shayan , Franziska Busch , Constance Aldinger , Constanze Buhrmann , Cora Lueders3, Ali Mobasheri4 1 Institute of Anatomy, Ludwig-Maximilian-University Munich, Munich, Germany, 2 Investigating Institute Molecular Biological System Transfer, Tehran, Iran, 3 Department of Thoracic and Cardiovascular Surgery, Laboratory for Tissue Engineering, German Heart Institute Berlin, Berlin, Germany, 4 Division of Veterinary Medicine, School of Veterinary Medicine and Science, Faculty of Medicine and Health Sciences, University of Nottingham, Sutton Bonington Campus, Sutton Bonington, United Kingdom Abstract Objective: Osteogenic repair in response to bone injury is characterized by activation and differentiation of mesenchymal stem cells (MSCs) to osteoblasts. This study determined whether activation of Sirt-1 (a NAD+-dependent histone deacetylase) by the phytoestrogen resveratrol affects osteogenic differentiation. Methods: Monolayer and high-density cultures of MSCs and pre-osteoblastic cells were treated with an osteogenic induction medium with/without the Sirt-1 inhibitor nicotinamide or/and resveratrol in a concentration dependent manner. Results: MSCs and pre-osteoblastic cells differentiated to osteoblasts when exposed to osteogenic-induction medium. The osteogenic response was blocked by nicotinamide, resulting in adipogenic differentiation and expression of the adipose transcription regulator PPAR-c (peroxisome proliferator-activated receptor). However, in nicotinamide-treated cultures, pre- tre