reverse and conventional chemical ecology approaches for the development of oviposition attractants for culex mosquitoes反向和常规化学生态学方法对库蚊蚊产卵引诱剂的发展.pdfVIP

Reverse and Conventional Chemical Ecology Approaches for the Development of Oviposition Attractants for Culex Mosquitoes 1 ˆ 2 1 1 1 1 Walter S. Leal *, Rosangela M. R. Barbosa , Wei Xu , Yuko Ishida , Zainulabeuddin Syed , Nicolas Latte , 1 1 3 ´ 2 Angela M. Chen , Tania I. Morgan , Anthony J. Cornel , Andre Furtado 1 Honorary Maeda-Duffey Laboratory, Department of Entomology, University of California Davis, Davis, California, United States of America, 2 Departamento de Entomologia, Centro de Pesquisas Ageu Magalhaes-Fiocruz, Recife, Brazil, 3 Mosquito Control Research Laboratory, Department of Entomology, University of California Davis, Davis, California, United States of America Abstract Synthetic mosquito oviposition attractants are sorely needed for surveillance and control programs for Culex species, which are major vectors of pathogens causing various human diseases, including filariasis, encephalitis, and West Nile encephalomyelitis. We employed novel and conventional chemical ecology approaches to identify potential attractants, which were demonstrated in field tests to be effective for monitoring populations of Cx. p. quinquefasciatus in human dwellings. Immunohistochemistry studies showed that an odorant-binding protein from this species, CquiOBP1, is expressed in trichoid sensilla on the antennae, including short, sharp-tipped trichoid sensilla type, which house an olfactory receptor neuron sensitive to a previously identified mosquito oviposition pheromone (MOP), 6-acetoxy-5-hexadecanolide. CquiOBP1 exists in monomeric and dime