rna-seq analysis reveals that an ecf σ factor, acss, regulates achromobactin biosynthesis in pseudomonas syringae pv. syringae b728arna-seq分析表明,ecfσ因子,acss,调节achromobactin丁香假单胞菌的生物合成。.pdfVIP

RNA-seq Analysis Reveals That an ECF s Factor, AcsS, Regulates Achromobactin Biosynthesis in Pseudomonas syringae pv. syringae B728a 1 1 2 2 1 Jessica W. Greenwald , Charles J. Greenwald , Benjamin J. Philmus , Tadhg P. Begley , Dennis C. Gross * 1 Department of Plant Pathology and Microbiology, Texas AM University, College Station, Texas, United States of America, 2 Department of Chemistry, Texas AM University, College Station, Texas, United States of America Abstract Iron is an essential micronutrient for Pseudomonas syringae pv. syringae strain B728a and many other microorganisms; therefore, B728a has evolved methods of iron acquirement including the use of iron-chelating siderophores. In this study an extracytoplasmic function (ECF) sigma factor, AcsS, encoded within the achromobactin gene cluster is shown to be a major regulator of genes involved in the biosynthesis and secretion of this siderophore. However, production of achromobactin was not completely abrogated in the deletion mutant, implying that other regulators may be involved such as PvdS, the sigma factor that regulates pyoverdine biosynthesis. RNA-seq analysis identified 287 genes that are differentially expressed between the AcsS deletion mutant and the wild type strain. These genes are involved in iron response, secretion, extracellular polysaccharide production, and cell motility. Thus, the transcriptome analysis supports a role for AcsS in the regulation of achromobactin production and the potential activity of both AcsS and achromobactin in the plant-associated lifestyle of strain B728a. Citation: Greenwald JW, Greenwald CJ, Philmus BJ, Begley TP, Gross DC (2012) RNA-seq Analysis Reveals