rna-seq atlas of glycine max a guide to the soybean transcriptomerna-seq地图集的大豆大豆转录组的指导.pdfVIP

Severin et al. BMC Plant Biology 2010, 10:160 /1471-2229/10/160 RESEARCH ARTICLE Open Access RNA-Seq Atlas of Glycine max: A guide to the soybean transcriptome 1* 1 2 1 3 4 Andrew J Severin , Jenna L Woody , Yung-Tsi Bolon , Bindu Joseph , Brian W Diers , Andrew D Farmer , 6 5 5 7 1,5 1,5 Gary J Muehlbauer , Rex T Nelson , David Grant , James E Specht , Michelle A Graham , Steven B Cannon , Gregory D May4, Carroll P Vance2,6, Randy C Shoemaker1,5 Abstract Background: Next generation sequencing is transforming our understanding of transcriptomes. It can determine the expression level of transcripts with a dynamic range of over six orders of magnitude from multiple tissues, developmental stages or conditions. Patterns of gene expression provide insight into functions of genes with unknown annotation. Results: The RNA Seq-Atlas presented here provides a record of high-resolution gene expression in a set of fourteen diverse tissues. Hierarchical clustering of transcriptional profiles for these tissues suggests three clades with similar profiles: aerial, underground and seed tissues. We also investigate the relationship between gene structure and gene expression and find a correlation between gene length and expression. Additionally, we find dramatic tissue-specific gene expression of both the most highly-expressed genes and the genes specific to legumes in seed development and nodule tissues. Analysis of the gene expression profiles of over 2,000 genes with preferential gene expression in seed suggests there are more than 177 genes with functional