rotavirus rearranged genomic rna segments are preferentially packaged into viruses despite not conferring selective growth advantage to viruses轮状病毒重组基因组rna片段优先包装成病毒尽管不是病毒赋予选择性生长优势.pdfVIP

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2017-09-11 发布于上海
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rotavirus rearranged genomic rna segments are preferentially packaged into viruses despite not conferring selective growth advantage to viruses轮状病毒重组基因组rna片段优先包装成病毒尽管不是病毒赋予选择性生长优势.pdf

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rotavirus rearranged genomic rna segments are preferentially packaged into viruses despite not conferring selective growth advantage to viruses轮状病毒重组基因组rna片段优先包装成病毒尽管不是病毒赋予选择性生长优势

Rotavirus Rearranged Genomic RNA Segments Are Preferentially Packaged into Viruses Despite Not Conferring Selective Growth Advantage to Viruses ´ 1. ´ 1,2,3. 1,2 1¤a 1¤b Cecile Troupin , Aurelie Schnuriger , Sarah Duponchel , Claire Deback , Nathalie Schnepf , Axelle Dehee1,3, Antoine Garbarg-Chenon1,2,3* ´ ´ 1 Micro-Organismes, Molecules Bioactives et Physiopathologie Intestinale, Universite Pierre et Marie Curie - Paris 6, Paris, France, 2 ERL U1057/UMR 7203, Institut ´ ´ ˆ ˆ National de la Sante et de la Recherche Medicale, Paris, France, 3 Laboratoire de Virologie, Hopital Armand Trousseau, Assistance Publique - Hopitaux de Paris, Paris, France Abstract The rotavirus (RV) genome consists of 11 double-stranded RNA segments. Sometimes, partial sequence duplication of an RNA segment leads to a rearranged RNA segment. To specify the impact of rearrangement, the replication efficiencies of human RV with rearranged segments 7, 11 or both were compared to these of the homologous human wild-type RV (wt-RV) and of the bovine wt-RV strain RF. As judged by viral growth curves, rotaviruses with a rearranged genome (r-RV) had no selective growth advantage over the homologous wt-RV. In contrast, r-RV were selected over wt-RV during competitive experiments (i.e mixed infections between r-RV and wt-RV followed by serial passages in cell culture). Moreover, when competitive experiments were performed between a human r-RV and