sampling strategy and potential utility of indels for dna barcoding of closely related plant species a case study in taxus的采样策略和潜在效用indels dna条码技术密切相关的植物物种紫杉的案例研究.pdfVIP

Int. J. Mol. Sci. 2012, 13, 8740-8751; doi:10.3390/ijm OPEN ACCESS International Journal of Molecular Sciences ISSN 1422-0067 /journal/ijms Article Sampling Strategy and Potential Utility of Indels for DNA Barcoding of Closely Related Plant Species: A Case Study in Taxus Jie Liu 1,2, Jim Provan 3, Lian-Ming Gao 1,* and De-Zhu Li 1,2,* 1 Key Laboratory of Biodiversity and Biogeography, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China; E-Mail: liujie@ 2 Plant Germplasm and Genomics Center, Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China 3 School of Biological Sciences, Queen’s University Belfast, 97 Lisburn Road, Belfast, BT9 7BL, UK; E-Mail: van@qub.ac.uk * Authors to whom correspondence should be addressed; E-Mails: gaolm@ (L.-M.G.); dzl@ (D.-Z.L.); Tel.: +86-871-5223505 (L.-M.G.); +86-871-5223503 (D.-Z.L.); Fax: +86-871-5217791 (D.-Z.L.). Received: 8 February 2012; in revised form: 23 June 2012 / Accepted: 27 June 2012 / Published: 13 July 2012 Abstract: Although DNA barcoding has become a useful tool for species identification and biodiversity surveys in plant sciences, there remains little consensus concerning appropriate sampling strategies and the treatment of indels. To address these two issues, we sampled 39 populations for nine Taxus species across their entire ranges, with two to three individuals per population randomly sampled. We sequenced