secretor genotype (fut2 gene) is strongly associated with the composition of bifidobacteria in the human intestine分泌腺基因型(fut2基因)与在人类肠道中双歧杆菌的成分.pdfVIP

Secretor Genotype (FUT2 gene) Is Strongly Associated with the Composition of Bifidobacteria in the Human Intestine ¨ ¨ ¨ ¨ Pirjo Wacklin*, Harri Makivuokko, Noora Alakulppi, Janne Nikkila, Heli Tenkanen, Jarkko Rabina, Jukka ¨ ¨ Partanen, Kari Aranko, Jaana Matto Finnish Red Cross Blood Service, Helsinki, Finland Abstract Intestinal microbiota plays an important role in human health, and its composition is determined by several factors, such as diet and host genotype. However, thus far it has remained unknown which host genes are determinants for the microbiota composition. We studied the diversity and abundance of dominant bacteria and bifidobacteria from the faecal samples of 71 healthy individuals. In this cohort, 14 were non-secretor individuals and the remainders were secretors. The secretor status is defined by the expression of the ABH and Lewis histo-blood group antigens in the intestinal mucus and other secretions. It is determined by fucosyltransferase 2 enzyme, encoded by the FUT2 gene. Non-functional enzyme resulting from a nonsense mutation in the FUT2 gene leads to the non-secretor phenotype. PCR-DGGE and qPCR methods were applied for the intestinal microbiota analysis. Principal component analysis of bifidobacterial DGGE profiles showed that the samples of non-secretor individuals formed a separate cluster within the secretor samples. Moreover, bifidobacterial diversity (p,0.0001), richness (p,0.0003), and abundance (p,0.05) were significantly reduced in the samples from the non- secretor individuals as compared with those from the secretor individuals. The non-secretor individuals lacked, or wer