securin is not required for chromosomal stability in human cellssecurin不需要在人类细胞中染色体的稳定性.pdfVIP

Open access, freely available online PLoS BIOLOGY Securin Is Not Required for Chromosomal Stability in Human Cells 1,2 3 ¨ 4 3 1,2* Katrin Pfleghaar , Simone Heubes , Jurgen Cox , Olaf Stemmann , Michael R. Speicher 1 Institute of Human Genetics, Technical University Munich, Munich, Germany, 2 Institute of Human Genetics, GSF National Research Center for Environment and Health, Neuherberg, Germany, 3 Department of Molecular Cell Biology, Max Planck Institute of Biochemistry, Martinsried, Germany, 4 Genedata GmbH, Martinsried, Germany Abnormalities of chromosome number are frequently observed in cancers. The mechanisms regulating chromosome segregation in human cells are therefore of great interest. Recently it has been reported that human cells without an hSecurin gene lose chromosomes at a high frequency. Here we show that, after hSecurin knockout through homologous recombination, chromosome losses are only a short, transient effect. After a few passages hSecurin/ cells became chromosomally stable and executed mitoses normally. This was unexpected, as the securin loss resulted in a persisting reduction of the sister-separating protease separase and inefficient cleavage of the cohesin subunit Scc1. Our data demonstrate that securin is dispensable for chromosomal stability in human cells. We propose that human cells possess efficient mechanisms to compensate for the loss of genes involved in chromosome segregation. Citation: Pfleghaar K, Heubes S, Cox J, Stemmann O, Speicher MR (2005) Securin is not required for chromosomal stability in human cells. PLoS Biol 3(12): e416. Introduction needed for chromosomal stability in human cells, as hS