selection of suitable reference genes for normalization of quantitative real-time pcr in cartilage tissue injury and repair in rabbits选择合适的参考正常化定量实时pcr基因在软骨组织损伤和修复的兔子.pdfVIP

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selection of suitable reference genes for normalization of quantitative real-time pcr in cartilage tissue injury and repair in rabbits选择合适的参考正常化定量实时pcr基因在软骨组织损伤和修复的兔子.pdf

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selection of suitable reference genes for normalization of quantitative real-time pcr in cartilage tissue injury and repair in rabbits选择合适的参考正常化定量实时pcr基因在软骨组织损伤和修复的兔子

Int. J. Mol. Sci. 2012, 13, 14344-14355; doi:10.3390/ijms131114344 OPEN ACCESS International Journal of Molecular Sciences ISSN 1422-0067 /journal/ijms Article Selection of Suitable Reference Genes for Normalization of Quantitative Real-Time PCR in Cartilage Tissue Injury and Repair in Rabbits Xiao-Xiang Peng 1,†, Rong-Lan Zhao 1,†, Wei Song 1, Hai-Rong Chu 1, Meng Li 1, Shu-Ya Song 1, Guang-Zhou Li 1 and Dong-Chun Liang 2,3,* 1 Shandong Provincial Key Laboratory of Clinical Laboratory Diagnostics, Department of Medical Laboratory, Weifang Medical University, Shandong 261053, China; E-Mails: pxx74@ (X.-X.P.); zhaoronglan@ (R.-L.Z.); wysw8103@163.com (W.S.); chr7968@163.com (H.-R.C.); 2006limeng@163.com (M.L.); shuyaxin2000@ (S.-Y.S.); lgz2006@ (G.-Z.L.) 2 Institute of Endocrinology and Metabolic Disease Hospital, Tianjin Medical University, Tianjin 300070, China 3 Key Lab of Hormone and Development, Tianjin Ministry, Tianjin 300070, China † These authors contributed equally to this work. * Author to whom correspondence should be addressed; E-Mail: dongchun.liang@; Tel.: +86-22-2337-4555; Fax: +86-22-2337-6177. Received: 4 August 2012; in revised form: 25 October 2012 / Accepted: 29 October 2012 / Published: 6 November 2012 Abstract: When studying the altered expression of genes associated with cartilage regeneration by quantitative real-time RT-PCR (RT-qPCR), reference genes with highly stable expression during different stage