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screen for localized proteins in caulobacter crescentus屏幕局部蛋白质茎菌属crescentus
Screen for Localized Proteins in Caulobacter crescentus
Jay H. Russell, Kenneth C. Keiler*
Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania, United States of America
Abstract
Precise localization of individual proteins is required for processes such as motility, chemotaxis, cell-cycle progression, and
cell division in bacteria, but the number of proteins that are localized in bacterial species is not known. A screen based on
transposon mutagenesis and fluorescence activated cell sorting was devised to identify large numbers of localized proteins,
and employed in Caulobacter crescentus. From a sample of the clones isolated in the screen, eleven proteins with no
previously characterized localization in C. crescentus were identified, including six hypothetical proteins. The localized
hypothetical proteins included one protein that was localized in a helix-like structure, and two proteins for which the
localization changed as a function of the cell cycle, suggesting that complex three-dimensional patterns and cell cycle-
dependent localization are likely to be common in bacteria. Other mutants produced localized fusion proteins even though
the transposon has inserted near the 5 9 end of a gene, demonstrating that short peptides can contain sufficient information
to localize bacterial proteins. The screen described here could be used in most bacterial species.
Citation: Russell JH, Keiler KC (2008) Screen for Localized Proteins in Caulobacter crescentus. PLoS ONE 3(3): e1756. doi:10.1371/journal.pone.0001756
Editor: Christophe Herman, Baylor College of Medicine, United States of America
Received December 7, 2007; Accepted February 7, 2008; Published March 12, 2008
Copyright: 2008 Russell, Keiler. This is an open-access article distributed unde
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