东亚飞蝗虫病原褪色沙雷氏菌的鉴定与检验-生物技术通报.pdfVIP

生物技术通报 ·研究报告· BIOTECHNOLOGY BULLETIN ( ) 2015, 31 12 :243-248 东亚飞蝗虫病原褪色沙雷氏菌的鉴定与检验 吴楠  郭杨柳  王晓珊  李艳云  卢会鹏  陈翠珍  张东林  房海 （河北科技师范学院，秦皇岛 066000） 摘 要 ： 病原褪色沙雷氏菌的有效分离与鉴定对控制由褪色沙雷氏菌引起的养殖东亚飞蝗感染病均具有一定的参考与应用 价值，同时将为对蝗灾的生物防治研究提供借鉴。对从发病死亡的养殖东亚飞蝗中分离的10 株细菌，进行了表观分类学指征、 16S rRNA 基因序列与系统发育、人工感染的致病作用等方面的检验与分析。结果表明为沙雷氏菌属的褪色沙雷氏菌，对供试养殖 东亚飞蝗显示强致病性。褪色沙雷氏菌对东亚飞蝗有强致病性，是蝗虫养殖的重要致病菌。 关键词 ： 东亚飞蝗；褪色沙雷氏菌 ；检验与分析 DOI ：10.13560/ki.biotech.bull.1985.2015.12.035 The Identification and Analysis of Pathogenic Serratia marcescens from Locusta migratoria manilensis Wu Nan Guo Yangliu Wang Xiaoshan Li Yanyun Lu Huipeng Chen Cuizhen Zhang Donglin Fang Hai （Hebei Normal University of Science and Technology ，Qinhuangdao 066000 ） Abstract: The effective isolation and identification of pathogen bacteria Serratia marcescens has a certain reference and application value for the control of infectious disease in culturing locusts caused by the S. marcescens. In addition, it will provide an insight for the research on biological control of plague of locust. From the dead Locusta migratoria manilensis from the infection, 10 strains of bacteria were isolated, and they were examined and analyzed with the apparent indications of taxonomy, 16S rRNA gene sequences and phylogenetic analysis, and artificial model of infection. Based on genetic and phenotypic information, the isolates were identified as S. marcescens which had high pathogenicity to cultured L. mingratoria manilensis. In conclusion, S. marcescens causes pathogenicity to L. mingratoria manilensis and it is a pathogenic bacterium in the culture of L. mingratoria manilensis, which lays a theoretical fou