藏羚羊组蛋白去乙酰化酶1基因编码区的克隆与序列分析-兽类学报.PDFVIP

兽类学报,2011,31 (4):396 - 403 Acta Theriologica Sinica 藏羚羊组蛋白去乙酰化酶 1 基因 编码区的克隆与序列分析 刘芳 格日力 (青海大学医学院高原医学研究中心,西宁 810001) 摘要:为探讨藏羚羊的低氧适应机制与高原低氧环境的相关性,采用 RT-PCR 技术,首次从藏羚羊心肌组织总 RNA 中克隆出组蛋白去乙酰化酶1 (Histone deacetylase1,HDAC1)基因的编码区序列。该序列全长为1 449 bp, 编码482 个氨基酸,预测其蛋白质分子量约为55 kDa。序列分析表明,藏羚羊 HDAC1 基因的编码区序列与其它 哺乳动物相似性超过90% ,其中与牛的相似性最高为 98 27% ;它的氨基酸序列与其它哺乳动物的相似性达到 98 76% ～ 99 59% ,显示出极高的保守性。利用邻接法 (Neighbor-Joining,NJ 法)构建的分子系统进化树聚类结 果表明,藏羚羊与牛先聚为一类,该聚类结果与传统的物种进化关系基本一致。藏羚羊 HDAC1 基因编码区的成 功获得,为进一步揭示藏羚羊低氧适应的表观遗传机制奠定基础。 关键词:藏羚羊;组蛋白去乙酰化酶 1;基因克隆;低氧适应 中图分类号:Q75 文献标识码:A 文章编号:1000 - 1050 (2011)04 - 0396 - 08 Cloning and sequencing of HDAC1 coding cDNA sequence from Tibetan antelope (Pantholops hodgsonii )  LIU Fang ,GE Rili (Research Center of High Altitude Medicine ,Medical College of Qinghai University,Xining 810001,China) Abstract :In order to explore the correlation between chronic hypoxia adaptation mechanism and a hypoxic environment at high altitude,the coding region of the HDAC1 gene was cloned for the first time from the total RNA of Tibetan antelope ’s cardiac muscle using RT-PCR. The encoding sequence was 1 449 bp in full length encoding 482 amino acids with a predic- ted molecular weight of about 55 kDa Sequence analysis showed that the coding region of the HDAC1 gene cloned from the total RNA of Tibetan antelope shared more than90% similarity with that of other mammals,in which the similarity reached as much as 98 27% . The amino acid sequence of HDAC1 of Tibetan antelope was 98 76% - 99 59% similar with that of other mammals,which was highly conservative. Cluster analysis of the molecular phylogenetic tree based on the HDCA1 gene sequences and