single-cell electric lysis on an electroosmotic-driven microfluidic chip with arrays of microwells单细胞电溶解在一个electroosmotic-driven microwells阵列微流控芯片.pdfVIP

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single-cell electric lysis on an electroosmotic-driven microfluidic chip with arrays of microwells单细胞电溶解在一个electroosmotic-driven microwells阵列微流控芯片.pdf

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single-cell electric lysis on an electroosmotic-driven microfluidic chip with arrays of microwells单细胞电溶解在一个electroosmotic-driven microwells阵列微流控芯片

Sensors 2012, 12, 6967-6977; doi:10.3390/s120606967 OPEN ACCESS sensors ISSN 1424-8220 /journal/sensors Article Single-Cell Electric Lysis on an Electroosmotic-Driven Microfluidic Chip with Arrays of Microwells Chun-Ping Jen 1,*, Tamara G. Amstislavskaya 2, Ya-Hui Liu 1, Ju-Hsiu Hsiao 1 and Yu-Hung Chen 3,* 1 Department of Mechanical Engineering, Advanced Institute of Manufacturing with High-Tech Innovation, National Chung Cheng University, Chia Yi, 62102, Taiwan; E-Mails: black_12_28@ (Y.-H.L.); ggina03@.tw (J.-H.H.) 2 Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, Novosibirsk, 630090, Russia; E-Mail: amst@bionet.nsc.ru 3 Department of Biochemistry and Molecular Biology, National Cheng-Kung University, Tainan, 70101, Taiwan * Authors to whom correspondence should be addressed; E-Mails: imecpj@.tw (C.-P.J.); second@.tw (Y.-H.C.); Tel.: +886-5-272-0411 (ext. 33322); Fax: +886-5-272-0589. Received: 16 April 2012; in revised form: 15 May 2012 / Accepted: 21 May 2012 / Published: 25 May 2012 Abstract: Accurate analysis at the single-cell level has become a highly attractive tool for investigating cellular content. An electroosmotic-driven microfluidic chip with arrays of 30-µm-diameter microwells was developed for single-cell electric lysis in the present study. The cellular occupancy in the microwells when the applied voltage was 5 V (82.4%) was slightly higher than that at an applied voltage of 10 V (81.8%). When the appli