snapshot of the eukaryotic gene expression in muskoxen rumen—a metatranscriptomic approach真核基因表达的快照muskoxen rumen-a metatranscriptomic方法.pdfVIP

Snapshot of the Eukaryotic Gene Expression in Muskoxen Rumen—A Metatranscriptomic Approach 1. 1,2. 3 4 1 4 Meng Qi , Pan Wang , Nicholas O’Toole , Perry S. Barboza , Emilio Ungerfeld , Mary Beth Leigh , 2 3 3 1 1 L. Brent Selinger , Greg Butler , Adrian Tsang , Tim A. McAllister , Robert J. Forster * 1 Lethbridge Research Centre, Agriculture and Agri-Food Canada, Lethbridge, Alberta, Canada, 2 Department of Biological Sciences, University of Lethbridge, Lethbridge, Alberta, Canada, 3 Centre for Structural and Functional Genomics, Concordia University, Montreal, Quebec, Canada, 4 Department of Biology and Wildlife, Institute of Arctic Biology, University of Alaska Fairbanks, Fairbanks, Alaska, United States of America Abstract Background: Herbivores rely on digestive tract lignocellulolytic microorganisms, including bacteria, fungi and protozoa, to derive energy and carbon from plant cell wall polysaccharides. Culture independent metagenomic studies have been used to reveal the genetic content of the bacterial species within gut microbiomes. However, the nature of the genes encoded by eukaryotic protozoa and fungi within these environments has not been explored using metagenomic or metatranscriptomic approaches. Methodology/Principal Findings: In this study, a metatranscriptomic approach was used to investigate the functional diversity of the eukaryotic microorganisms within the rumen of muskoxen (Ovibos moschatus), with a focus on plant cell wall degrading enzymes. Polyadenylated RNA (mRNA) was sequenced on the Illumina Genome Analyzer II system and 2.8 g