specific nuclear envelope transmembrane proteins can promote the location of chromosomes to and from the nuclear periphery特定的核被膜跨膜蛋白可以促进染色体的位置和核外围.pdfVIP

Specific nuclear envelope transmembrane proteins can promote the location of chromosomes to and from the nuclear periphery Zuleger et al. Zuleger et al. Genome Biology 2013, 14:R14 /2013/14/2/R14 (15 February 2013) Zuleger et al. Genome Biology 2013, 14:R14 /2013/14/2/R14 RESEARCH Open Access Specific nuclear envelope transmembrane proteins can promote the location of chromosomes to and from the nuclear periphery 1 2 1 1 1 1 Nikolaj Zuleger , Shelagh Boyle , David A Kelly , Jose I de las Heras , Vassiliki Lazou , Nadia Korfali , 1 3,4 3,4 5 2 Dzmitry G Batrakou , K Natalie Randles , Glenn E Morris , David J Harrison , Wendy A Bickmore and Eric C Schirmer1* Abstract Background: Different cell types have distinctive patterns of chromosome positioning in the nucleus. Although ectopic affinity-tethering of specific loci can be used to relocate chromosomes to the nuclear periphery, endogenous nuclear envelope proteins that control such a mechanism in mammalian cells have yet to be widely identified. Results: To search for such proteins, 23 nuclear envelope transmembrane proteins were screened for their ability to promote peripheral localization of human chromosomes in HT1080 fibroblasts. Five of these proteins had strong effects on chromosome 5, but individual proteins affected different subsets of chromosomes. The repositioning effects were reversible and the proteins with effects all exhibited highly tissue-restricted patterns of expression. Depletion