staphylococcus aureus keratinocyte invasion is dependent upon multiple high-affinity fibronectin-binding repeats within fnbpa金黄色葡萄球菌角化细胞入侵取决于多个高亲和性fibronectin-binding fnbpa内重复.pdfVIP

Staphylococcus aureus Keratinocyte Invasion Is Dependent upon Multiple High-Affinity Fibronectin- Binding Repeats within FnBPA 1 2 3 3,4 1 Andrew M. Edwards *, Ursula Potter , Nicola A. G. Meenan , Jennifer R. Potts , Ruth C. Massey 1 Department of Biology and Biochemistry, University of Bath, Bath, United Kingdom, 2 Microscopic Analysis Suite, University of Bath, Bath, United Kingdom, 3 Department of Biology, University of York, York, United Kingdom, 4 Department of Chemistry, University of York, York, United Kingdom Abstract Staphylococcus aureus is a commensal organism and a frequent cause of skin and soft tissue infections, which can progress to serious invasive disease. This bacterium uses its fibronectin binding proteins (FnBPs) to invade host cells and it has been hypothesised that this provides a protected niche from host antimicrobial defences, allows access to deeper tissues and provides a reservoir for persistent or recurring infections. FnBPs contain multiple tandem fibronectin-binding repeats (FnBRs) which bind fibronectin with varying affinity but it is unclear what selects for this configuration. Since both colonisation and skin infection are dependent upon the interaction of S. aureus with keratinocytes we hypothesised that this might select for FnBP function and thus composition of the FnBR region. Initial experiments revealed that S. aureus attachment to keratinocytes is rapid but does not require FnBRs. By contrast, invasion of keratinocytes was dependent upon the FnBR region and occurred via similar cellular processes to those described for endothelial cells. Despite this, keratinocyte invasion was relative