study of active ingredients in black soybean sprouts and their safety in cosmetic use黑大豆萌芽活性成分的研究及其在化妆品安全使用.pdfVIP

Molecules 2012, 17, 11669-11679; doi:10.3390/molecules171011669 OPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article Study of Active Ingredients in Black Soybean Sprouts and Their Safety in Cosmetic Use 1 2 3 1 4 4 1 Jixiang Lai , Can Xin , Ya Zhao , Bing Feng , Congfen He , Yinmao Dong , Yun Fang and Shaomin Wei 1,3,* 1 School of Chemical and Material Engineering, Jiangnan University, Wuxi 214122, Jiangsu, China 2 School of Chemical Engineering, Hebei United University, Tangshan 063009, Hebei, China 3 Shanghai Jahwa United Co. Ltd, Shanghai 200082, China 4 School of Science, Beijing Technology and Business University, Beijing 100048, China * Author to whom correspondence should be addressed; E-Mail: weishaomin@; Tel.: +86-021-2501-2302; Fax: +86-021-6976-8460. Received: 10 July 2012; in revised form: 13 September 2012 / Accepted: 19 September 2012 / Published: 1 October 2012 Abstract: Active ingredients in different lengths of black soybean sprouts were extracted with water. Concentrations of the main proteins and polysaccharides were determined by the Forint phenol assay and phenol-sulfuric acid assay, respectively. Anti-oxidizing capacities of the extracts were measured in vitro using the DPPH scavenging test and whitening capacity was measured in vitro using the tyrosinase inhibition test. The effects of the bean sprout extracts on human skin fibroblasts damnified